| Title | [Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC] | | Author(s) | He FY, Zhou HH, Luo JY, Deng KW, Fu J, Pi FJ, Wu DZ, Dai RW | | Institution | Pharmacogenetic Research Institute, Central South University, Changsha 410078, China. pharmsharking@tom.com | | Source | Zhongguo Zhong Yao Za Zhi 2008 Mar; 33(6):642-8. | | Abstract | OBJECTIVE: To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC.
METHOD: The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL.
RESULT: The mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28.31 J(1/2) cm(-3/2) in accordance with 28.84 J(1/2) cm(-3/2) reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39.70 J(1/2) cm(-3/2), 39.08 J(1/2) cm(-3/2), 38.37 J(1/2) cm(-3/2), 36.42 J(1/2) cm(-3/2) respectively.
CONCLUSION: The retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 18590190 |
|
